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LearnNB300-537
antibody from Novus Biologicals
Targeting: PPARA
hPPAR, NR1C1, PPAR
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Gel shift Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [6]
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- Product number
- NB300-537 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-537, RRID:AB_10002535
- Product name
- Mouse Monoclonal PPAR alpha/NR1C1 Antibody
- Antibody type
- Monoclonal
- Description
- Immunogen affinity purified. PPAR alpha (3B6/PPAR)
- Reactivity
- Human, Mouse, Rat, Bovine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Hypoxia training improves hepatic steatosis partly by downregulation of CB1 receptor in obese mice.
Augmenter of liver regeneration protein deficiency promotes hepatic steatosis by inducing oxidative stress and microRNA-540 expression.
Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway.
Pharmacological inhibition of NOX4 ameliorates alcohol-induced liver injury in mice through improving oxidative stress and mitochondrial function.
Expression of Peroxisome Proliferator-Activated Receptor alpha (PPARα) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate in GH3 cells.
Modulating Intrafollicular Hormonal Milieu in Controlled Ovarian Stimulation: Insights From PPAR Expression in Human Granulosa Cells.
The expression and role of peroxisome proliferator-activated receptor α in atherosclerosis.
Expression of the cannabinoid system in muscle: effects of a high-fat diet and CB1 receptor blockade.
A mutant thyroid hormone receptor alpha antagonizes peroxisome proliferator-activated receptor alpha signaling in vivo and impairs fatty acid oxidation.
Inhibition of matrix metalloproteinases increases PPAR-alpha and IL-6 and prevents dietary-induced hepatic steatosis and injury in a murine model.
Evidence that peroxisome proliferator-activated receptor alpha is complexed with the 90-kDa heat shock protein and the hepatitis virus B X-associated protein 2.
Yang Q, Sun S, Liu W, Liu Q, Wang J
Biochemical and biophysical research communications 2020 May 7;525(3):639-645
Biochemical and biophysical research communications 2020 May 7;525(3):639-645
Augmenter of liver regeneration protein deficiency promotes hepatic steatosis by inducing oxidative stress and microRNA-540 expression.
Kumar S, Rani R, Karns R, Gandhi CR
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Mar;33(3):3825-3840
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Mar;33(3):3825-3840
Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway.
Li L, He M, Xiao H, Liu X, Wang K, Zhang Y
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(5):2021-2030
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(5):2021-2030
Pharmacological inhibition of NOX4 ameliorates alcohol-induced liver injury in mice through improving oxidative stress and mitochondrial function.
Sun Q, Zhang W, Zhong W, Sun X, Zhou Z
Biochimica et biophysica acta. General subjects 2017 Jan;1861(1 Pt A):2912-2921
Biochimica et biophysica acta. General subjects 2017 Jan;1861(1 Pt A):2912-2921
Expression of Peroxisome Proliferator-Activated Receptor alpha (PPARα) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate in GH3 cells.
Rotondi S, Modarelli A, Oliva MA, Rostomyan L, Sanita P, Ventura L, Daly AF, Esposito V, Angelucci A, Arcella A, Giangaspero F, Beckers A, Jaffrain-Rea ML
Molecular and cellular endocrinology 2016 May 5;426:61-72
Molecular and cellular endocrinology 2016 May 5;426:61-72
Modulating Intrafollicular Hormonal Milieu in Controlled Ovarian Stimulation: Insights From PPAR Expression in Human Granulosa Cells.
Tatone C, Benedetti E, Vitti M, Di Emidio G, Ciriminna R, Vento ME, Cela V, Borzì P, Carta G, Lispi M, Cimini AM, Artini PG, Italian Society of Embryology, Reproduction and Research (SIERR).
Journal of cellular physiology 2016 Apr;231(4):908-14
Journal of cellular physiology 2016 Apr;231(4):908-14
The expression and role of peroxisome proliferator-activated receptor α in atherosclerosis.
Gao W, Xu W, Li H
Molecular medicine reports 2013 Mar;7(3):862-8
Molecular medicine reports 2013 Mar;7(3):862-8
Expression of the cannabinoid system in muscle: effects of a high-fat diet and CB1 receptor blockade.
Crespillo A, Suárez J, Bermúdez-Silva FJ, Rivera P, Vida M, Alonso M, Palomino A, Lucena MA, Serrano A, Pérez-Martín M, Macias M, Fernández-Llébrez P, Rodríguez de Fonseca F
The Biochemical journal 2011 Jan 1;433(1):175-85
The Biochemical journal 2011 Jan 1;433(1):175-85
A mutant thyroid hormone receptor alpha antagonizes peroxisome proliferator-activated receptor alpha signaling in vivo and impairs fatty acid oxidation.
Liu YY, Heymann RS, Moatamed F, Schultz JJ, Sobel D, Brent GA
Endocrinology 2007 Mar;148(3):1206-17
Endocrinology 2007 Mar;148(3):1206-17
Inhibition of matrix metalloproteinases increases PPAR-alpha and IL-6 and prevents dietary-induced hepatic steatosis and injury in a murine model.
Alwayn IP, Andersson C, Lee S, Arsenault DA, Bistrian BR, Gura KM, Nose V, Zauscher B, Moses M, Puder M
American journal of physiology. Gastrointestinal and liver physiology 2006 Dec;291(6):G1011-9
American journal of physiology. Gastrointestinal and liver physiology 2006 Dec;291(6):G1011-9
Evidence that peroxisome proliferator-activated receptor alpha is complexed with the 90-kDa heat shock protein and the hepatitis virus B X-associated protein 2.
Sumanasekera WK, Tien ES, Turpey R, Vanden Heuvel JP, Perdew GH
The Journal of biological chemistry 2003 Feb 14;278(7):4467-73
The Journal of biological chemistry 2003 Feb 14;278(7):4467-73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - 293T cell lysate. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Jurkat cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Hela cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in 3T3 cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Jurkat cells with PPAR alpha/NR1C1 Antibody (3B6/PPAR) NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Hek293 cells with PPAR alpha/NR1C1 [3B6/PPAR] Antibody NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
