Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX103747 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103747, RRID:AB_11171647
- Product name
- USP8 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Post-translational control of T cell development by the ESCRT protein CHMP5.
Adoro S, Park KH, Bettigole SE, Lis R, Shin HR, Seo H, Kim JH, Knobeloch KP, Shim JH, Glimcher LH
Nature immunology 2017 Jul;18(7):780-790
Nature immunology 2017 Jul;18(7):780-790
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: H1299 B: HCT116 5% SDS PAGE GTX103747 diluted at 1:5000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- USP8 antibody detects USP8 protein at cytoplasm by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: USP8 protein stained by USP8 antibody (GTX103747) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of USP8 protein from HCT-116 whole cell extracts using 5 £gg of USP8 antibody (GTX103747).Western blot analysis was performed using USP8 antibody (GTX103747).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using USP8(GTX103747) antibody at 1:500 dilution.
