Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-79048 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CIS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Identification of a MicroRNA-E3 Ubiquitin Ligase Regulatory Network for Hepatocyte Death in Alcohol-Associated Hepatitis.
Fan X, Wu J, Poulsen KL, Kim A, Wu X, Huang E, Miyata T, Sanz-Garcia C, Nagy LE
Hepatology communications 2021 May;5(5):830-845
Hepatology communications 2021 May;5(5):830-845
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CIS in Lane 1: rat liver tissue lysate, Lane 2: rat kidney tissue lysate, Lane 3: human placenta tissue lysate, Lane 4: A431 whole cell lysate, Lane 5: SMMC whole cell lysate, Lane 6: HeLa whole cell lysate, Lane 7: COLO320 whole cell lysate, Lane 8: MM231 whole cell lysate using 40-50 µg per well. Sample was incubated with CIS (Product # PA5-79048) at a dilution of 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CIS in Lane 1: rat liver tissue lysate, Lane 2: rat kidney tissue lysate, Lane 3: human placenta tissue lysate, Lane 4: A431 whole cell lysate, Lane 5: SMMC whole cell lysate, Lane 6: HeLa whole cell lysate, Lane 7: COLO320 whole cell lysate, Lane 8: MM231 whole cell lysate using 40-50 µg per well. Sample was incubated with CIS (Product # PA5-79048) at a dilution of 0.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CIS on paraffin-embedded rat kidney tissue. Sample was incubated with CIS polyclonal antibody (Product# PA5-79048).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CIS on paraffin-embedded human lung cancer tissue. Sample was incubated with CIS polyclonal antibody (Product# PA5-79048).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 6 Fig. miR-150-5p directly targets CISH. (A) Predicted target site of miR-150-5p in 3'UTR region of CISH mRNA and sequence alignment between the mouse 3'UTR-CISH and human 3'UTR-CISH by BLAST. The dots represent target sites, and the vertical lines indicate similarity between human and murine sequences. (B) AML12 cells were transiently transfected with miR-150-5p mimic, inhibitor, or miRNA negative control (200 nM) for 48 hours, and protein levels of CISH were determined by western blot (n = 3). (C) The luciferase reporter plasmid containing CISH 3'UTR was co-transfected with miR-150-5p mimic, inhibitor, or miRNA negative control (200 nM) in AML12 cells. Luciferase activities were determined at 24 hours after transfection. The relative luciferase activity was normalized to the miRNA negative control group. Values represent means +- SEM, and P values are presented at the top of the bar graphs.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Increased miR-150-5p and decreased CISH were identified in patients with AH and the Gao-Binge mice model. Expression of miR-150-5p was determined in human livers (A) and mice livers (B) using quantitative real-time PCR and normalized to RNU6. Data are represented as fold change of EtOH versus pair group (n = 5-7 for each group). Expression of CISH was determined in human livers (C) and mice livers (D) using quantitative real-time PCR and normalized to 18 seconds. Data are represented as fold change of EtOH versus pair group (n = 5-7 for each group). (E) Expression of CISH protein was measured in the livers of patients with AH and heathy individuals by western blot (n = 5 for each group). Densitometry was carried out with HSC70 as a loading control. (F) Expression of CISH protein was measured in the livers of mice by western blot (n = 4-5 for each group). Densitometry was carried out with beta-actin as a loading control. Values represent means +- SEM, and P values are presented at the top of the bar graphs.