PA5-28959
antibody from Invitrogen Antibodies
Targeting: UBE2D1
E2(17)KB1, SFT, UBC4/5, UBCH5, UbcH5A
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-28959 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE2D1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (95%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using UBE2D1 Polyclonal Antibody (Product # PA5-28959). Sample (30 µg of whole cell lysate). A: A431. 10% SDS PAGE. UBE2D1 Polyclonal Antibody (Product # PA5-28959) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of UBE2D1 in paraformaldehyde-fixed A431 cells using a UBE2D1 polyclonal antibody (Product # PA5-28959) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using UBE2D1 (Product # PA5-28959) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- UBE2D1 Polyclonal Antibody detects UBE2D1 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. UBE2D1 Polyclonal Antibody (Product # PA5-28959) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- UBE2D1 Polyclonal Antibody detects UBE2D1 protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. UBE2D1 Polyclonal Antibody (Product # PA5-28959) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.