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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF1730 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Integrin beta 2/CD18 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Integrin beta 2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin beta 1, recombinant mouse Integrin beta 2, rhIntegrin beta 3, rhIntegrin beta 4, rhIntegrin beta 5, rhIntegrin beta 6, and rhIntegrin beta 7 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAA59490- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Hypoxia and matrix viscoelasticity sequentially regulate endothelial progenitor cluster-based vasculogenesis.
Biomimetic post-capillary venule expansions for leukocyte adhesion studies.
Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities.
Bystander cells enhance NK cytotoxic efficiency by reducing search time.
An anti-inflammatory property of Candida albicans β-glucan: Induction of high levels of interleukin-1 receptor antagonist via a Dectin-1/CR3 independent mechanism.
Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells.
Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation.
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
Blatchley MR, Hall F, Wang S, Pruitt HC, Gerecht S
Science advances 2019 Mar;5(3):eaau7518
Science advances 2019 Mar;5(3):eaau7518
Biomimetic post-capillary venule expansions for leukocyte adhesion studies.
Benson BL, Li L, Myers JT, Dorand RD, Gurkan UA, Huang AY, Ransohoff RM
Scientific reports 2018 Jun 19;8(1):9328
Scientific reports 2018 Jun 19;8(1):9328
Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities.
Lavoie SS, Dumas E, Vulesevic B, Neagoe PE, White M, Sirois MG
Journal of immunology (Baltimore, Md. : 1950) 2018 Jun 1;200(11):3801-3813
Journal of immunology (Baltimore, Md. : 1950) 2018 Jun 1;200(11):3801-3813
Bystander cells enhance NK cytotoxic efficiency by reducing search time.
Zhou X, Zhao R, Schwarz K, Mangeat M, Schwarz EC, Hamed M, Bogeski I, Helms V, Rieger H, Qu B
Scientific reports 2017 Mar 13;7:44357
Scientific reports 2017 Mar 13;7:44357
An anti-inflammatory property of Candida albicans β-glucan: Induction of high levels of interleukin-1 receptor antagonist via a Dectin-1/CR3 independent mechanism.
Smeekens SP, Gresnigt MS, Becker KL, Cheng SC, Netea SA, Jacobs L, Jansen T, van de Veerdonk FL, Williams DL, Joosten LA, Dinarello CA, Netea MG
Cytokine 2015 Feb;71(2):215-22
Cytokine 2015 Feb;71(2):215-22
Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells.
Dotterweich J, Ebert R, Kraus S, Tower RJ, Jakob F, Schütze N
Cell communication and signaling : CCS 2014 Jun 25;12:36
Cell communication and signaling : CCS 2014 Jun 25;12:36
Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation.
Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, Kettritz R
The Journal of biological chemistry 2011 Mar 4;286(9):7070-81
The Journal of biological chemistry 2011 Mar 4;286(9):7070-81
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Digestive diseases and sciences 2007 Oct;52(10):2775-83
Digestive diseases and sciences 2007 Oct;52(10):2775-83
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Integrin beta 2/CD18 in Human PBMCs. Integrin beta 2/CD18 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human Integrin beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
