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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- GTX15786 - Provider product page
- Provider
- GeneTex
- Product name
- CX3CL1 antibody [4C5]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of CX3CL1 in 0.25 £gg of recombinant human CX3CL1 protein. Protein was transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membrane was probed with CX3CL1 antibody [4C5] at a dilution of 1:1000 overnight at 4¢XC on a rocking platform. Membranes were washed in TBS-0.1% Tween-20 and probed with a HRP-conjugated secondary antibody. Membrane was washed and chemiluminescent detection was performed.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELISA analysis of human CX3CL1 was performed by coating a blank 96-well microtiter plate with 1£gg/ml of recombinant CX3CL1 protein and incubating overnight. The plate was aspirated and blocked with block solution. The plate was washed then incubated for 2 hours with serial dilutions (10£gg/ml) of CX3CL1 antibody [4C5]. The plate was washed and incubated with a proper secondary antibody, washed again, and 100£gl per well of TMB substrate was added for 30 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.
