Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF537 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat CX3CL1/Fractalkine Chemokine Domain Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat CX3CL1/Fractalkine in ELISAs and Western blots. Neutralizes the biological activity of recombinant human CX3CL1 and recombinant mouse CX3CL1 with similar effectiveness.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O55145
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Noradrenaline induces CX3CL1 production and release by neurons.
Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats.
Selective activation of microglia facilitates synaptic strength.
Interactions between chemokines: regulation of fractalkine/CX3CL1 homeostasis by SDF/CXCL12 in cortical neurons.
Inhibition of spinal microglial cathepsin S for the reversal of neuropathic pain.
Madrigal JL, Caso JR, García-Bueno B, Gutiérrez IL, Leza JC
Neuropharmacology 2017 Mar 1;114:146-155
Neuropharmacology 2017 Mar 1;114:146-155
Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats.
Yeh PT, Huang HW, Yang CM, Yang WS, Yang CH
PloS one 2016;11(1):e0146438
PloS one 2016;11(1):e0146438
Selective activation of microglia facilitates synaptic strength.
Clark AK, Gruber-Schoffnegger D, Drdla-Schutting R, Gerhold KJ, Malcangio M, Sandkühler J
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Mar 18;35(11):4552-70
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Mar 18;35(11):4552-70
Interactions between chemokines: regulation of fractalkine/CX3CL1 homeostasis by SDF/CXCL12 in cortical neurons.
Cook A, Hippensteel R, Shimizu S, Nicolai J, Fatatis A, Meucci O
The Journal of biological chemistry 2010 Apr 2;285(14):10563-71
The Journal of biological chemistry 2010 Apr 2;285(14):10563-71
Inhibition of spinal microglial cathepsin S for the reversal of neuropathic pain.
Clark AK, Yip PK, Grist J, Gentry C, Staniland AA, Marchand F, Dehvari M, Wotherspoon G, Winter J, Ullah J, Bevan S, Malcangio M
Proceedings of the National Academy of Sciences of the United States of America 2007 Jun 19;104(25):10655-60
Proceedings of the National Academy of Sciences of the United States of America 2007 Jun 19;104(25):10655-60
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CX3CL1/Fractalkine in Mouse Brain. CX3CL1/Fractalkine was detected in perfusion fixed frozen sections of mouse brain (cingulate cortex) using 1.7 µg/mL Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Rat CX3CL1/Fractalkine Antibody. Recombinant Rat CX3CL1/Fractalkine (Catalog # 536-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CX3CL1/Fractalkine (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537). The ND50 is typically 0.3-1.2 µg/mL.