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- Antibody Data
- Antigen structure
- References [20]
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- Blocking/Neutralizing [1]
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- Product number
- AF365 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CX3CL1/Fractalkine Chemokine Domain Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CX3CL1/Fractalkine in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) MCP-2 and rhMCP-4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q6I9S9- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Placental CX3CL1 is Deregulated by Angiotensin II and Contributes to a Pro-Inflammatory Trophoblast-Monocyte Interaction.
The Fractalkine-Receptor Axis Improves Human Colorectal Cancer Prognosis by Limiting Tumor Metastatic Dissemination.
Fractalkine-CX3CR1-dependent recruitment and retention of human CD1c+ myeloid dendritic cells by in vitro-activated proximal tubular epithelial cells.
Involvement of the CX3CL1 (fractalkine)/CX3CR1 pathway in the pathogenesis of acute graft-versus-host disease.
Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy.
Expression and regulation of chemokines in murine and human type 1 diabetes.
Fractalkine is a novel human adipochemokine associated with type 2 diabetes.
Thromboxane prostanoid receptor stimulation induces shedding of the transmembrane chemokine CX3CL1 yet enhances CX3CL1-dependent leukocyte adhesion.
An engineered CX3CR1 antagonist endowed with anti-inflammatory activity.
Constitutive endocytosis of the chemokine CX3CL1 prevents its degradation by cell surface metalloproteases.
CX3CR1 is expressed by prostate epithelial cells and androgens regulate the levels of CX3CL1/fractalkine in the bone marrow: potential role in prostate cancer bone tropism.
Fractalkine and CX3CR1 mediate leukocyte capture by endothelium in response to Shiga toxin.
Fractalkine and CX3CR1 mediate leukocyte capture by endothelium in response to Shiga toxin.
Fractalkine, a CX3C chemokine, as a mediator of ocular angiogenesis.
Down-regulation of surface fractalkine by RNA interference in B16 melanoma reduced tumor growth in mice.
Immunohistochemical assessment of fractalkine, inflammatory cells, and human herpesvirus 7 in human salivary glands.
Human cytomegalovirus-specific CD4+-T-cell cytokine response induces fractalkine in endothelial cells.
Detection of fractalkine in human seminal plasma and its role in infertile patients.
Detection of fractalkine in human seminal plasma and its role in infertile patients.
The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo.
Nonn O, Güttler J, Forstner D, Maninger S, Zadora J, Balogh A, Frolova A, Glasner A, Herse F, Gauster M
International journal of molecular sciences 2019 Feb 2;20(3)
International journal of molecular sciences 2019 Feb 2;20(3)
The Fractalkine-Receptor Axis Improves Human Colorectal Cancer Prognosis by Limiting Tumor Metastatic Dissemination.
Erreni M, Siddiqui I, Marelli G, Grizzi F, Bianchi P, Morone D, Marchesi F, Celesti G, Pesce S, Doni A, Rumio C, Roncalli MG, Laghi L, Mantovani A, Allavena P
Journal of immunology (Baltimore, Md. : 1950) 2016 Jan 15;196(2):902-14
Journal of immunology (Baltimore, Md. : 1950) 2016 Jan 15;196(2):902-14
Fractalkine-CX3CR1-dependent recruitment and retention of human CD1c+ myeloid dendritic cells by in vitro-activated proximal tubular epithelial cells.
Kassianos AJ, Wang X, Sampangi S, Afrin S, Wilkinson R, Healy H
Kidney international 2015 Jun;87(6):1153-63
Kidney international 2015 Jun;87(6):1153-63
Involvement of the CX3CL1 (fractalkine)/CX3CR1 pathway in the pathogenesis of acute graft-versus-host disease.
Brissot E, Bossard C, Malard F, Braudeau C, Chevallier P, Guillaume T, Delaunay J, Josien R, Gregoire M, Gaugler B, Mohty M
Journal of leukocyte biology 2015 Feb;97(2):227-35
Journal of leukocyte biology 2015 Feb;97(2):227-35
Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy.
Wang Y, Wei Q, Liu Q, Li Z, Zhou L, Zou F, Yuan Y, Sun Z
Molecular medicine reports 2013 Dec;8(6):1817-23
Molecular medicine reports 2013 Dec;8(6):1817-23
Expression and regulation of chemokines in murine and human type 1 diabetes.
Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
Diabetes 2012 Feb;61(2):436-46
Diabetes 2012 Feb;61(2):436-46
Fractalkine is a novel human adipochemokine associated with type 2 diabetes.
Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP
Diabetes 2011 May;60(5):1512-8
Diabetes 2011 May;60(5):1512-8
Thromboxane prostanoid receptor stimulation induces shedding of the transmembrane chemokine CX3CL1 yet enhances CX3CL1-dependent leukocyte adhesion.
Tole S, Durkan AM, Huang YW, Liu GY, Leung A, Jones LL, Taylor JA, Robinson LA
American journal of physiology. Cell physiology 2010 Jun;298(6):C1469-80
American journal of physiology. Cell physiology 2010 Jun;298(6):C1469-80
An engineered CX3CR1 antagonist endowed with anti-inflammatory activity.
Dorgham K, Ghadiri A, Hermand P, Rodero M, Poupel L, Iga M, Hartley O, Gorochov G, Combadière C, Deterre P
Journal of leukocyte biology 2009 Oct;86(4):903-11
Journal of leukocyte biology 2009 Oct;86(4):903-11
Constitutive endocytosis of the chemokine CX3CL1 prevents its degradation by cell surface metalloproteases.
Huang YW, Su P, Liu GY, Crow MR, Chaukos D, Yan H, Robinson LA
The Journal of biological chemistry 2009 Oct 23;284(43):29644-53
The Journal of biological chemistry 2009 Oct 23;284(43):29644-53
CX3CR1 is expressed by prostate epithelial cells and androgens regulate the levels of CX3CL1/fractalkine in the bone marrow: potential role in prostate cancer bone tropism.
Jamieson WL, Shimizu S, D'Ambrosio JA, Meucci O, Fatatis A
Cancer research 2008 Mar 15;68(6):1715-22
Cancer research 2008 Mar 15;68(6):1715-22
Fractalkine and CX3CR1 mediate leukocyte capture by endothelium in response to Shiga toxin.
Zanchi C, Zoja C, Morigi M, Valsecchi F, Liu XY, Rottoli D, Locatelli M, Buelli S, Pezzotta A, Mapelli P, Geelen J, Remuzzi G, Hawiger J
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):1460-9
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):1460-9
Fractalkine and CX3CR1 mediate leukocyte capture by endothelium in response to Shiga toxin.
Zanchi C, Zoja C, Morigi M, Valsecchi F, Liu XY, Rottoli D, Locatelli M, Buelli S, Pezzotta A, Mapelli P, Geelen J, Remuzzi G, Hawiger J
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):1460-9
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):1460-9
Fractalkine, a CX3C chemokine, as a mediator of ocular angiogenesis.
You JJ, Yang CH, Huang JS, Chen MS, Yang CM
Investigative ophthalmology & visual science 2007 Nov;48(11):5290-8
Investigative ophthalmology & visual science 2007 Nov;48(11):5290-8
Down-regulation of surface fractalkine by RNA interference in B16 melanoma reduced tumor growth in mice.
Ren T, Chen Q, Tian Z, Wei H
Biochemical and biophysical research communications 2007 Dec 28;364(4):978-84
Biochemical and biophysical research communications 2007 Dec 28;364(4):978-84
Immunohistochemical assessment of fractalkine, inflammatory cells, and human herpesvirus 7 in human salivary glands.
Latchney LR, Fallon MA, Culp DJ, Gelbard HA, Dewhurst S
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2004 May;52(5):671-81
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2004 May;52(5):671-81
Human cytomegalovirus-specific CD4+-T-cell cytokine response induces fractalkine in endothelial cells.
Bolovan-Fritts CA, Trout RN, Spector SA
Journal of virology 2004 Dec;78(23):13173-81
Journal of virology 2004 Dec;78(23):13173-81
Detection of fractalkine in human seminal plasma and its role in infertile patients.
Zhang Q, Shimoya K, Ohta Y, Chin R, Tenma K, Isaka S, Nakamura H, Koyama M, Azuma C, Murata Y
Human reproduction (Oxford, England) 2002 Jun;17(6):1560-4
Human reproduction (Oxford, England) 2002 Jun;17(6):1560-4
Detection of fractalkine in human seminal plasma and its role in infertile patients.
Zhang Q, Shimoya K, Ohta Y, Chin R, Tenma K, Isaka S, Nakamura H, Koyama M, Azuma C, Murata Y
Human reproduction (Oxford, England) 2002 Jun;17(6):1560-4
Human reproduction (Oxford, England) 2002 Jun;17(6):1560-4
The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo.
Lucas AD, Chadwick N, Warren BF, Jewell DP, Gordon S, Powrie F, Greaves DR
The American journal of pathology 2001 Mar;158(3):855-66
The American journal of pathology 2001 Mar;158(3):855-66
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CX3CL1/Fractalkine in Human Breast Cancer Tissue. CX3CL1/Fractalkine was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF365) overnight at 4 °C, using an antibody diluent at pH 9.5-10.5. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Human CX3CL1/Fractalkine Antibody. Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF365). The ND50 is typically 0.75-4.5 µg/mL.
