NB100-368
antibody from Novus Biologicals
Targeting: UPF1
HUPF1, KIAA0221, NORF1, pNORF1, RENT1, smg-2
Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NB100-368 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-368, RRID:AB_2213199
- Product name
- Goat Polyclonal RENT1/UPF1/hUPF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Stabilization of lncRNA GAS5 by a Small Molecule and Its Implications in Diabetic Adipocytes.
Interaction of PABPC1 with the translation initiation complex is critical to the NMD resistance of AUG-proximal nonsense mutations.
Cotranscriptional effect of a premature termination codon revealed by live-cell imaging.
Sam68 regulates EMT through alternative splicing-activated nonsense-mediated mRNA decay of the SF2/ASF proto-oncogene.
Role of the RNA-binding protein IMP-2 in muscle cell motility.
Nonsense-mediated mRNA decay effectors are essential for zebrafish embryonic development and survival.
Ultraconserved elements are associated with homeostatic control of splicing regulators by alternative splicing and nonsense-mediated decay.
A post-transcriptional regulatory switch in polypyrimidine tract-binding proteins reprograms alternative splicing in developing neurons.
DNA-activated protein kinase functions in a newly observed S phase checkpoint that links histone mRNA abundance with DNA replication.
The canonical UPF1-dependent nonsense-mediated mRNA decay is inhibited in transcripts carrying a short open reading frame independent of sequence context.
Shi Y, Parag S, Patel R, Lui A, Murr M, Cai J, Patel NA
Cell chemical biology 2019 Mar 21;26(3):319-330.e6
Cell chemical biology 2019 Mar 21;26(3):319-330.e6
Interaction of PABPC1 with the translation initiation complex is critical to the NMD resistance of AUG-proximal nonsense mutations.
Peixeiro I, Inácio Â, Barbosa C, Silva AL, Liebhaber SA, Romão L
Nucleic acids research 2012 Feb;40(3):1160-73
Nucleic acids research 2012 Feb;40(3):1160-73
Cotranscriptional effect of a premature termination codon revealed by live-cell imaging.
de Turris V, Nicholson P, Orozco RZ, Singer RH, Mühlemann O
RNA (New York, N.Y.) 2011 Dec;17(12):2094-107
RNA (New York, N.Y.) 2011 Dec;17(12):2094-107
Sam68 regulates EMT through alternative splicing-activated nonsense-mediated mRNA decay of the SF2/ASF proto-oncogene.
Valacca C, Bonomi S, Buratti E, Pedrotti S, Baralle FE, Sette C, Ghigna C, Biamonti G
The Journal of cell biology 2010 Oct 4;191(1):87-99
The Journal of cell biology 2010 Oct 4;191(1):87-99
Role of the RNA-binding protein IMP-2 in muscle cell motility.
Boudoukha S, Cuvellier S, Polesskaya A
Molecular and cellular biology 2010 Dec;30(24):5710-25
Molecular and cellular biology 2010 Dec;30(24):5710-25
Nonsense-mediated mRNA decay effectors are essential for zebrafish embryonic development and survival.
Wittkopp N, Huntzinger E, Weiler C, Saulière J, Schmidt S, Sonawane M, Izaurralde E
Molecular and cellular biology 2009 Jul;29(13):3517-28
Molecular and cellular biology 2009 Jul;29(13):3517-28
Ultraconserved elements are associated with homeostatic control of splicing regulators by alternative splicing and nonsense-mediated decay.
Ni JZ, Grate L, Donohue JP, Preston C, Nobida N, O'Brien G, Shiue L, Clark TA, Blume JE, Ares M Jr
Genes & development 2007 Mar 15;21(6):708-18
Genes & development 2007 Mar 15;21(6):708-18
A post-transcriptional regulatory switch in polypyrimidine tract-binding proteins reprograms alternative splicing in developing neurons.
Boutz PL, Stoilov P, Li Q, Lin CH, Chawla G, Ostrow K, Shiue L, Ares M Jr, Black DL
Genes & development 2007 Jul 1;21(13):1636-52
Genes & development 2007 Jul 1;21(13):1636-52
DNA-activated protein kinase functions in a newly observed S phase checkpoint that links histone mRNA abundance with DNA replication.
Müller B, Blackburn J, Feijoo C, Zhao X, Smythe C
The Journal of cell biology 2007 Dec 31;179(7):1385-98
The Journal of cell biology 2007 Dec 31;179(7):1385-98
The canonical UPF1-dependent nonsense-mediated mRNA decay is inhibited in transcripts carrying a short open reading frame independent of sequence context.
Silva AL, Pereira FJ, Morgado A, Kong J, Martins R, Faustino P, Liebhaber SA, Romão L
RNA (New York, N.Y.) 2006 Dec;12(12):2160-70
RNA (New York, N.Y.) 2006 Dec;12(12):2160-70
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-368] - Whole cell lysate (30 ug) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody used at 1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-368] - Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-RENT1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.