HPA052171

antibody from Atlas Antibodies

Targeting: GPI AMF, NLK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA052171

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA052171, RRID:AB_2681748

Product name

Anti-GPI

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSN
IDGTHIAKTLAQLNPESSLFIIASKTFTTQETITN
AETAKEWFLQAAKDPSAVAKHFVALST


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell line A-549 and human cell line SK-MEL-30.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-GPI antibody HPA052171 (A) shows similar pattern to independent antibody HPA024305 (B).

Antibody #2 product nr

HPA024305

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts..

Sample type

HUMAN