H00002821-D01

antibody from Abnova Corporation

Targeting: GPI AMF, NLK

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002821-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002821-D01, RRID:AB_10659343

Product name

GPI MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human GPI protein.

Antigen sequence

MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKD
RFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVD
LAKSRGVEAARERMFNGEKINYTEGRAVLHVALRN
RSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGD
WKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYS
SGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIAS
KTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVA
LSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAI
GLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKN
APVLLALLGIWYINCFGCETHAMLPYDQYLHRFAA
YFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGT
NGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGL
HHKILLANFLAQTEALMRGKSTEEARKELQAAGKS
PEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGAL
VAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKI
EPELDGSAQVTSHDASTNGLINFIKQQREARVQ


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GPI MaxPab rabbit polyclonal antibody. Western Blot analysis of GPI expression in human pancreas.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GPI MaxPab rabbit polyclonal antibody. Western Blot analysis of GPI expression in HeLa.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of GPI expression in transfected 293T cell line (H00002821-T03) by GPI MaxPab polyclonal antibody.Lane 1: GPI transfected lysate(63.10 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of GPI transfected lysate using anti-GPI MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GPI purified MaxPab mouse polyclonal antibody (B02P) (H00002821-B02P).

Validation comment

Immunoprecipitation

Protocol

Protocol