15171-1-AP

antibody from Invitrogen Antibodies

Targeting: GPI AMF, NLK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15171-1-AP

Provider

Invitrogen Antibodies

Product name

GPI Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAALTRDPQ FQKLQQWYRE HRSELNLRRL FDANKDRFNH FSLTLNTNHG HILVDYSKNL VTEDVMRMLV DLAKSRGVEA ARERMFNGEK INYTEGRAVL HVALRNRSNT PILVDGKDVM PEVNKVLDKM KSFCQRVRSG DWKGYTGKTI TDVINIGIGG SDLGPLMVTE ALKPYSSGGP RVWYVSNIDG THIAKTLAQL NPESSLFIIA SKTFTTQETI TNAETAKEWF LQAAKDPSAV AKHFVALSTN TTKVKEFGID PQNMFEFWDW VGG (1-272 aa encoded by BC004982)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.22 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed PC-3 cells using 15171-1-AP (GPI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 15171-1-AP ( GPI Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 15171-1-AP ( GPI Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-GPI (IP:15171-1-AP, 4ug; Detection:15171-1-AP 1:500) with HeLa cells lysate 1200ug.