H00004190-M01

antibody from Abnova Corporation

Targeting: MDH1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004190-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004190-M01, RRID:AB_425542

Product name

MDH1 monoclonal antibody (M01), clone 2B11-B7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant MDH1.

Antigen sequence

MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQP
IILVLLDITPMMGVLDGVLMELQDCALPLLKDVIA
TDKEDVAFKDLDVAILVGSMPRREGMERKDLLKAN
VKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTA
SKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTA
NDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVY
EALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMS
AAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGV
PDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDL
TAKELTEEKESAFEFLSSA


Isotype

IgG

Antibody clone number

2B11-B7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MDH1 monoclonal antibody (M01), clone 2B11-B7. Western Blot analysis of MDH1 expression in HepG2 ( Cat # L019V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MDH1 monoclonal antibody (M01), clone 2B11-B7. Western Blot analysis of MDH1 expression in HL-60 ( Cat # L014V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MDH1 expression in transfected 293T cell line by MDH1 monoclonal antibody (M01), clone 2B11-B7.Lane 1: MDH1 transfected lysate(36.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MDH1 is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol