MA5-44922

antibody from Invitrogen Antibodies

Targeting: SLC7A11 xCT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-44922

Provider

Invitrogen Antibodies

Product name

SLC7A11 Recombinant Mouse Monoclonal Antibody (A7C6-R)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

A7C6-R

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of xCT in different lysates (10 µg/Lane). Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in xCT Monoclonal antibody (Product # MA5-44922) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Mouse IgG - HRP secondary antibody at a dilution of 1:100,000 for 1 hour at room temperature. Lane 1: HT-29 cell lysate; Lane 2: A549 cell lysate. Predicted band size: 55 kDa. Observed band size: 55 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of xCT in HCT116 cell lysates (10 µg/Lane). Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in xCT Monoclonal antibody (Product # MA5-44922) using a dilution of 1:5,000 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Mouse IgG - HRP secondary antibody at a dilution of 1:100,000 for 1 hour at room temperature. Predicted band size: 55 kDa. Observed band size: 55 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of xCT in HT-29 cells. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Samples were incubated in xCT Monoclonal antibody (Product # MA5-44922) using a dilution of 1:200 in 2% BSA overnight at 4 ℃ followed by Goat Anti-Mouse IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of xCT in paraffin-embedded human pancreas tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with xCT Monoclonal antibody (Product # MA5-44922) using a dilution of 1:600 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of xCT in A549 cells. Cells were washed twice with cold PBS and resuspend then stained with xCT Monoclonal antibody (Product # MA5-44922) using a dilution of 1 µg/mL (red) at 4℃ for 30 minutes followed by iFluor™ 488 conjugate-Goat anti-Mouse IgG secondary antibody at a dilution of 1:1,000 for 30 minutes at 4℃. Mouse IgG1 Isotype Control (green). Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of xCT in HT-29 cells. Cells were washed twice with cold PBS and resuspend then stained with xCT Monoclonal antibody (Product # MA5-44922) using a dilution of 1 µg/mL (red) at 4℃ for 30 minutes followed by iFluor™ 488 conjugate-Goat anti-Mouse IgG secondary antibody at a dilution of 1:1,000 for 30 minutes at 4℃. Mouse IgG1 Isotype Control (green). Unlabeled sample was used as a control (cells without incubation with primary antibody; black).