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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 12-5846-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TdT Monoclonal Antibody (19-3), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 19-3 monoclonal antibody recognizes human and mouse terminal-deoxynucleotidyl transferase (TdT), a 60 kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes. Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. Tdt plays a critical role during TCR and Ig gene rearrangement. Regulation of expression in the mouse thymus correlates with T cell selection; decreased or absent after positive selection. Additionally Tdt has been shown to be present in lymphoma or lymphoblastic leukemia cells.
- Conjugate
- Yellow dye
- Antibody clone number
- 19-3
- Concentration
- 0.2 mg/mL
Submitted references The mitochondrial iron transporter ABCB7 is required for B cell development, proliferation, and class switch recombination in mice.
V(D)J recombination process and the Pre-B to immature B-cells transition are altered in Fanca(-/-) mice.
Regulation of N-region diversity in antigen receptors through thymocyte differentiation and thymus ontogeny.
Detection of terminal deoxynucleotidyl transferase (TdT) by flow cytometry in leukemic disorders.
Immunological detection of a conserved structure for terminal deoxynucleotidyltransferase.
Lehrke MJ, Shapiro MJ, Rajcula MJ, Kennedy MM, McCue SA, Medina KL, Shapiro VS
eLife 2021 Nov 11;10
eLife 2021 Nov 11;10
V(D)J recombination process and the Pre-B to immature B-cells transition are altered in Fanca(-/-) mice.
Nguyen TV, Pawlikowska P, Firlej V, Rosselli F, Aoufouchi S
Scientific reports 2016 Nov 24;6:36906
Scientific reports 2016 Nov 24;6:36906
Regulation of N-region diversity in antigen receptors through thymocyte differentiation and thymus ontogeny.
Bogue M, Gilfillan S, Benoist C, Mathis D
Proceedings of the National Academy of Sciences of the United States of America 1992 Nov 15;89(22):11011-5
Proceedings of the National Academy of Sciences of the United States of America 1992 Nov 15;89(22):11011-5
Detection of terminal deoxynucleotidyl transferase (TdT) by flow cytometry in leukemic disorders.
Bardales RH, Carrato A, Fleischer M, Schwartz MK, Koziner B
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1989 Apr;37(4):509-13
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1989 Apr;37(4):509-13
Immunological detection of a conserved structure for terminal deoxynucleotidyltransferase.
Bollum FJ, Chang LM
The Journal of biological chemistry 1981 Aug 25;256(16):8767-70
The Journal of biological chemistry 1981 Aug 25;256(16):8767-70
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of C57BL/6 thymocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) and Anti-Mouse CD8a PerCP-Cy5-5 (Product # 45-0081-82) followed by fixation and permeabilization with the Foxp3 Staining Buffers (Product # 00-5523-00) and subsequent staining with 0.25 µg of Anti-Mouse TdT PE. CD4 single positives are shown in gray, CD8 single positives in green and double positives in purple.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. Gene expression changes confirm absence of pre-B cells in Mb1-cre ABCB7 conditional knockout (cKO) mice. Analysis of critical transcription factors in wild-type (WT) and Mb1-cre ABCB7 cKO Fr. C cells (B220 + CD19 + CD43 + BP-1 + ). ( A-G ) Intracellular flow cytometry analysis of EBF1 ( A ), E47 (E2A) ( B ), FOXO1 ( C ), PAX5 ( D ), IKAROS ( E ), AIOLOS ( F ), and IRF4 ( G ) expression. Quantification of MdFI is shown on the right of each plot. Isotype controls are shown in gray. Offset histograms are representative of at least three independent experiments (total of 6-10 mice/group). ( H, I ) Flow cytometry analysis of CD2 ( H ) and CD25 ( I ) expression. Indicated values are the proportion of Fr. C cells positive for either marker, and quantifications are shown on the right of each plot. Offset histograms are representative of three independent experiments (total of five mice/group). ( J ) Intracellular flow cytometry analysis of TdT expression in Fr. B and Fr. C cells. Indicated values are the proportion of cells positive for TdT expression, and quantifications are shown on the right. Offset histograms are representative of three independent experiments (total of 5-7 mice/group). ( K ) Quantitative real-time PCR analysis of Rag1 and Rag2 expression in sorted Fr. B and Fr. C cells. 18S rRNA was used as an endogenous control, and relative expression values were normalized to expression in WT Fr. B cells. Results were obtained from three independent experiments (tot
- Conjugate
- Yellow dye
