MA5-27683

antibody from Invitrogen Antibodies

Targeting: KCNC2 Kv3.2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA5-27683

Provider

Invitrogen Antibodies

Product name

Anti-KV3.2 (KCNC2) Monoclonal Antibody (S410-17)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S410-17

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KV3.2 (KCNC2) in mouse, rat brain with 20 µg of sample. The sample was incubated with KV3.2 (KCNC2) monoclonal antibody (Product # MA5-27683) using a dilution of 1:1000 (16 hours at 4°C), followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:200 and ECL development (5 min at RT). Samples were arranged as follows: Lane 1: MW Ladder, Lane 2: Mouse Brain, Lane 3: Rat Brain membrane, Lane 4: Rat Brain.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KV3.2 (KCNC2) in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with KV3.2 (KCNC2) monoclonal antibody (Product # MA5-27683) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Kv3,2 Antibody, D) Merged image. Magnification: 60x.