AMAb90740

antibody from Atlas Antibodies

Targeting: REST DFNA27, NRSF, XBR

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMAb90740

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb90740, RRID:AB_2665651

Product name

Anti-REST

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

SPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVG
AEEADESLPGLAANINESTHISSSGQNLNTPEGET
LNGKHQTDSIVCEMKMDTDQNTRENLTGINSTVEE
PVSPMLPPSAVEEREAVSKTALA


Epitope

Binds to an epitope located within the peptide sequence ISSSGQNLNT as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL0381

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of U-2 OS cells using the Anti-REST monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows strong nuclear immunoreactivity in the lymphoid cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows strong nuclear immunoreactivity in the epithelial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong nuclear positivity in the seminiferous ducts.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows nuclear immunoreactivity in both renal tubuli and glomeruli.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cortex shows absence of staining in the neuronal nuclei (negative control).

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows absence of staining in hepatocytes (negative control).

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows moderate to strong nuclear positivity in lymphoid cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows moderate nuclear positivity in glandular and stromal cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows no nuclear positivity in neuronal cells as expected.