GTX129144

antibody from GeneTex

Targeting: REST DFNA27, NRSF, XBR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX129144

Provider

GeneTex

Product name

REST antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

REST antibody detects REST protein by western blot analysis.A. 30 £gg HeLa whole cell lysate/extract B. 30 £gg HeLa nuclear lysate/extract5 % SDS-PAGEREST antibody (GTX129144) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

U87-MG whole cell and nuclear extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with REST antibody (GTX129144) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and REST knockout (KO) HeLa cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with REST antibody (GTX129144) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and REST knockout (KO) HeLa cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with REST antibody (GTX129144) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

REST antibody detects REST protein at cytoplasm and nucleus in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. REST antibody (GTX129144) diluted at 1:400.