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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 22242-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- REST Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: ELSPPMEVV QKEPVKIELS PPIEVVQKEP VQMELSPPMG VVQKEPAQRE PPPPREPPLH MEPISKKPPL RKDKKEKSNM QSERARKEQV LIEVGLVPVK DSWLLKESVS TEDLSPPSPP LPKENLREEA SGDQKLLNTG EGNKEAPLQK VGAEEADESL PGLAANINES THISSSGQNL NTPEGETLNG KHQTDSIVCE MKMDTDQNTR ENLTGINSTV EEPVSPMLPP SAVEEREAVS KTALASPPAT MAANESQEID EDEGIHSHEG SDLSDNMSEG SDDSGLHGAR PVPQESSRKN AKEALAVKAA KGDFVCIFCD RSFRKGKDYS KHLNRHLVNV YYLEEAAQGQ E (748-1097 aa encoded by BC132859)
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 22242-1-AP (REST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 22242-1-AP (REST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of REST antibody (22242-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REST transfected HEK-293 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-REST (IP:22242-1-AP, 5ug; Detection:22242-1-AP 1:1000) with HeLa cells lysate 2720ug.
