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Western blot
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- Antibody Data
- Antigen structure
- References [19]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- MAB901 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MMP-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects pro and active forms of human MMP-1. In Western blots, no cross-reactivity with recombinant human (rh) MMP-2, rhMMP-3, or rhMMP-9 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P03956- Isotype
- IgG
- Antibody clone number
- 36665
- Vial size
- 200 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Protective effects of Camellia japonica flower extract against urban air pollutants.
The expression of MMP-1 and MMP-9 is up-regulated by smooth muscle cells after their cross-talk with macrophages in high glucose conditions.
Co-treatment of TGF-β3 and BMP7 is superior in stimulating chondrocyte redifferentiation in both hypoxia and normoxia compared to single treatments.
The Effects of the WNT-Signaling Modulators BIO and PKF118-310 on the Chondrogenic Differentiation of Human Mesenchymal Stem Cells.
Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression.
Matrix metalloproteinase-9 activity and a downregulated Hedgehog pathway impair blood-brain barrier function in an in vitro model of CNS tuberculosis.
MLK3 regulates FRA-1 and MMPs to drive invasion and transendothelial migration in triple-negative breast cancer cells.
MMP-1 and Pro-MMP-10 as potential urinary pharmacodynamic biomarkers of FGFR3-targeted therapy in patients with bladder cancer.
Myoferlin depletion in breast cancer cells promotes mesenchymal to epithelial shape change and stalls invasion.
Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
Matrix metalloproteinase activity in pediatric acute lung injury.
Matrix metalloproteinase activity in pediatric acute lung injury.
Effect of bimatoprost, latanoprost, and unoprostone on matrix metalloproteinases and their inhibitors in human ciliary body smooth muscle cells.
Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
Interleukin-1beta induced activation of nuclear factor-kappab can be inhibited by novel pharmacological agents in osteoarthritis.
The association of Sam68 with Vav1 contributes to tumorigenesis.
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
c-Kit immunophenotyping and metalloproteinase expression profiles of mast cells in interstitial lung diseases.
Kim M, Son D, Shin S, Park D, Byun S, Jung E
BMC complementary and alternative medicine 2019 Jan 28;19(1):30
BMC complementary and alternative medicine 2019 Jan 28;19(1):30
The expression of MMP-1 and MMP-9 is up-regulated by smooth muscle cells after their cross-talk with macrophages in high glucose conditions.
Macarie RD, Vadana M, Ciortan L, Tucureanu MM, Ciobanu A, Vinereanu D, Manduteanu I, Simionescu M, Butoi E
Journal of cellular and molecular medicine 2018 Sep;22(9):4366-4376
Journal of cellular and molecular medicine 2018 Sep;22(9):4366-4376
Co-treatment of TGF-β3 and BMP7 is superior in stimulating chondrocyte redifferentiation in both hypoxia and normoxia compared to single treatments.
Huang X, Zhong L, Post JN, Karperien M
Scientific reports 2018 Jul 6;8(1):10251
Scientific reports 2018 Jul 6;8(1):10251
The Effects of the WNT-Signaling Modulators BIO and PKF118-310 on the Chondrogenic Differentiation of Human Mesenchymal Stem Cells.
Huang X, Zhong L, Hendriks J, Post JN, Karperien M
International journal of molecular sciences 2018 Feb 13;19(2)
International journal of molecular sciences 2018 Feb 13;19(2)
Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression.
Han AR, Lim TG, Song YR, Jang M, Rhee YK, Hong HD, Kim MH, Kim HJ, Cho CW
International journal of molecular sciences 2018 Aug 24;19(9)
International journal of molecular sciences 2018 Aug 24;19(9)
Matrix metalloproteinase-9 activity and a downregulated Hedgehog pathway impair blood-brain barrier function in an in vitro model of CNS tuberculosis.
Brilha S, Ong CWM, Weksler B, Romero N, Couraud PO, Friedland JS
Scientific reports 2017 Nov 22;7(1):16031
Scientific reports 2017 Nov 22;7(1):16031
MLK3 regulates FRA-1 and MMPs to drive invasion and transendothelial migration in triple-negative breast cancer cells.
Rattanasinchai C, Llewellyn BJ, Conrad SE, Gallo KA
Oncogenesis 2017 Jun 12;6(6):e345
Oncogenesis 2017 Jun 12;6(6):e345
MMP-1 and Pro-MMP-10 as potential urinary pharmacodynamic biomarkers of FGFR3-targeted therapy in patients with bladder cancer.
Du X, Lin BC, Wang QR, Li H, Ingalla E, Tien J, Rooney I, Ashkenazi A, Penuel E, Qing J
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Dec 15;20(24):6324-35
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Dec 15;20(24):6324-35
Myoferlin depletion in breast cancer cells promotes mesenchymal to epithelial shape change and stalls invasion.
Li R, Ackerman WE 4th, Mihai C, Volakis LI, Ghadiali S, Kniss DA
PloS one 2012;7(6):e39766
PloS one 2012;7(6):e39766
Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
Cavallo C, Desando G, Facchini A, Grigolo B
Journal of biomedical materials research. Part A 2010 Apr;93(1):86-95
Journal of biomedical materials research. Part A 2010 Apr;93(1):86-95
Matrix metalloproteinase activity in pediatric acute lung injury.
Kong MY, Gaggar A, Li Y, Winkler M, Blalock JE, Clancy JP
International journal of medical sciences 2009;6(1):9-17
International journal of medical sciences 2009;6(1):9-17
Matrix metalloproteinase activity in pediatric acute lung injury.
Kong MY, Gaggar A, Li Y, Winkler M, Blalock JE, Clancy JP
International journal of medical sciences 2009;6(1):9-17
International journal of medical sciences 2009;6(1):9-17
Effect of bimatoprost, latanoprost, and unoprostone on matrix metalloproteinases and their inhibitors in human ciliary body smooth muscle cells.
Ooi YH, Oh DJ, Rhee DJ
Investigative ophthalmology & visual science 2009 Nov;50(11):5259-65
Investigative ophthalmology & visual science 2009 Nov;50(11):5259-65
Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
Villeneuve J, Block A, Le Bousse-Kerdilès MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
Experimental hematology 2009 Jul;37(7):849-56
Experimental hematology 2009 Jul;37(7):849-56
Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
Naveau A, Reinald N, Fournier B, Durand E, Lafont A, Coulomb B, Gogly B
Connective tissue research 2007;48(6):300-8
Connective tissue research 2007;48(6):300-8
Interleukin-1beta induced activation of nuclear factor-kappab can be inhibited by novel pharmacological agents in osteoarthritis.
Lauder SN, Carty SM, Carpenter CE, Hill RJ, Talamas F, Bondeson J, Brennan P, Williams AS
Rheumatology (Oxford, England) 2007 May;46(5):752-8
Rheumatology (Oxford, England) 2007 May;46(5):752-8
The association of Sam68 with Vav1 contributes to tumorigenesis.
Lazer G, Pe'er L, Schapira V, Richard S, Katzav S
Cellular signalling 2007 Dec;19(12):2479-86
Cellular signalling 2007 Dec;19(12):2479-86
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
Gut 2006 Dec;55(12):1774-80
Gut 2006 Dec;55(12):1774-80
c-Kit immunophenotyping and metalloproteinase expression profiles of mast cells in interstitial lung diseases.
Edwards ST, Cruz AC, Donnelly S, Dazin PF, Schulman ES, Jones KD, Wolters PJ, Hoopes C, Dolganov GM, Fang KC
The Journal of pathology 2005 Jul;206(3):279-90
The Journal of pathology 2005 Jul;206(3):279-90
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MMP-1 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMP-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human MMP-1 Specificity by Using Knockout Cell Line. Western blot shows lysates of PC-3 human prostate cancer parental cell line and MMP-1 knockout PC-3 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-1 in Human Ovarian Array. MMP-1 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-1 in Human Ovarian Cancer Tissue. MMP-1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
