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- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ABIN953466 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) (AA 314-349), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 314-349 amino acids from the Central region of Human MMP-1
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 314-349,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Raf kinase inhibitor protein suppresses nuclear factor-κB-dependent cancer cell invasion through negative regulation of matrix metalloproteinase expression.
Beshir AB, Ren G, Magpusao AN, Barone LM, Yeung KC, Fenteany G
Cancer letters 2010 Dec 28;299(2):137-49
Cancer letters 2010 Dec 28;299(2):137-49
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of MMP1 Antibody (Center) Cat.-No AP52712PU-N in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the MMP1 antibody detected the MMP1 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of MMP1 Antibody (Center) Cat.-No AP52712PU-N with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma reacted with MMP1 Antibody (Center) Cat.-No AP52712PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
