PAB9924

antibody from Abnova Corporation

Targeting: ATR FRP1, MEC1, SCKL, SCKL1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB9924

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB9924, RRID:AB_1672163

Product name

ATR polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of ATR.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint.

Bruno T, De Nicola F, Iezzi S, Lecis D, D'Angelo C, Di Padova M, Corbi N, Dimiziani L, Zannini L, Jekimovs C, Scarsella M, Porrello A, Chersi A, Crescenzi M, Leonetti C, Khanna KK, Soddu S, Floridi A, Passananti C, Delia D, Fanciulli M
Cancer cell 2006 Dec;10(6):473-86

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RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint.

Olson E, Nievera CJ, Klimovich V, Fanning E, Wu X
The Journal of biological chemistry 2006 Dec 22;281(51):39517-33

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ATR-dependent phosphorylation and activation of ATM in response to UV treatment or replication fork stalling.

Stiff T, Walker SA, Cerosaletti K, Goodarzi AA, Petermann E, Concannon P, O'Driscoll M, Jeggo PA
The EMBO journal 2006 Dec 13;25(24):5775-82

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using ATR polyclonal antibody (Cat # PAB9924) in HeLa cell nuclear extract (Lane 1).Lane 2 shows negligible staining after pre-incubation of antibody with the immunizing peptide (50 ug peptide for 1 h at room temperature followed by centrifugation).A 4-8% gradient gel was used for separation.Goat serum was used at 5% for blocking.The arrowhead corresponds to 301 kDa ATR.The primary antibody was used at a 1 : 1,400 dilution.