GTX101777

antibody from GeneTex

Targeting: FEN1 FEN-1, MF1, RAD2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

GTX101777

Provider

GeneTex

Proper citation

GeneTex Cat#GTX101777, RRID:AB_1950269

Product name

FEN1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Inhibition of hydrogen sulfide biosynthesis sensitizes lung adenocarcinoma to chemotherapeutic drugs by inhibiting mitochondrial DNA repair and suppressing cellular bioenergetics.

Szczesny B, Marcatti M, Zatarain JR, Druzhyna N, Wiktorowicz JE, Nagy P, Hellmich MR, Szabo C
Scientific reports 2016 Nov 3;6:36125

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using FEN1(GTX101777) antibody at 1:100 dilution.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 10% SDS PAGE GTX101777 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FEN1 antibody detects FEN1 protein by western blot analysis. A. 30 ?g NIH-3T3 whole cell lysate/extract B. 30 ?g JC whole cell lysate/extract C. 30 ?g BCL-1 whole cell lysate/extract D. 30 ?g Raw264.7 whole cell lysate/extract 10% SDS-PAGE FEN1 antibody (GTX101777) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FEN1 antibody detects FEN1 protein by western blot analysis. A. 30 ?g PC-12 whole cell lysate/extract 10% SDS-PAGE FEN1 antibody (GTX101777) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed A549, using FEN1(GTX101777) antibody at 1:200 dilution.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FEN1 antibody immunoprecipitates FEN1 protein in IP experiments.IP samples: Jurkat whole cell extractA. 30 £gg Jurkat whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of FEN1 protein by 4 £gg FEN1 antibody (GTX101777)10 % SDS-PAGEThe immunoprecipitated FEN1 protein was detected by FEN1 antibody (GTX101777) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FEN1 antibody detects FEN1 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. FEN1 antibody (GTX101777) diluted at 1:500.