GTX70185

antibody from GeneTex

Targeting: FEN1 FEN-1, MF1, RAD2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX70185

Provider

GeneTex

Proper citation

GeneTex Cat#GTX70185, RRID:AB_370522

Product name

FEN1 antibody [4E7]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

References

Submitted references

Mechanisms of Targeting the MDM2-p53-FOXM1 Axis in Well-Differentiated Intestinal Neuroendocrine Tumors.

Briest F, Grass I, Sedding D, Möbs M, Christen F, Benecke J, Fuchs K, Mende S, Kaemmerer D, Sänger J, Kunze A, Geisler C, Freitag H, Lewens F, Worpenberg L, Iwaszkiewicz S, Siegmund B, Walther W, Hummel M, Grabowski P
Neuroendocrinology 2018;107(1):1-23

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hDNA2 nuclease/helicase promotes centromeric DNA replication and genome stability.

Li Z, Liu B, Jin W, Wu X, Zhou M, Liu VZ, Goel A, Shen Z, Zheng L, Shen B
The EMBO journal 2018 Jul 13;37(14)

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Overexpression of Flap Endonuclease 1 Correlates with Enhanced Proliferation and Poor Prognosis of Non-Small-Cell Lung Cancer.

Zhang K, Keymeulen S, Nelson R, Tong TR, Yuan YC, Yun X, Liu Z, Lopez J, Raz DJ, Kim JY
The American journal of pathology 2018 Jan;188(1):242-251

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Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage.

Tsai YC, Wang YH, Liu YC
Journal of nucleic acids 2017;2017:8154646

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RECQ1 interacts with FEN-1 and promotes binding of FEN-1 to telomeric chromatin.

Sami F, Lu X, Parvathaneni S, Roy R, Gary RK, Sharma S
The Biochemical journal 2015 Jun 1;468(2):227-44

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A cryptic targeting signal creates a mitochondrial FEN1 isoform with tailed R-Loop binding properties.

Kazak L, Reyes A, He J, Wood SR, Brea-Calvo G, Holen TT, Holt IJ
PloS one 2013;8(5):e62340

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Apoptosis induced by persistent single-strand breaks in mitochondrial genome: critical role of EXOG (5'-EXO/endonuclease) in their repair.

Tann AW, Boldogh I, Meiss G, Qian W, Van Houten B, Mitra S, Szczesny B
The Journal of biological chemistry 2011 Sep 16;286(37):31975-83

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Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding.

Guo Z, Zheng L, Xu H, Dai H, Zhou M, Pascua MR, Chen QM, Shen B
Nature chemical biology 2010 Oct;6(10):766-73

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DNA polymerase beta-dependent long patch base excision repair in living cells.

Asagoshi K, Liu Y, Masaoka A, Lan L, Prasad R, Horton JK, Brown AR, Wang XH, Bdour HM, Sobol RW, Taylor JS, Yasui A, Wilson SH
DNA repair 2010 Feb 4;9(2):109-19

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DNA polymerase beta and PARP activities in base excision repair in living cells.

Masaoka A, Horton JK, Beard WA, Wilson SH
DNA repair 2009 Nov 2;8(11):1290-9

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Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells.

Kong X, Mohanty SK, Stephens J, Heale JT, Gomez-Godinez V, Shi LZ, Kim JS, Yokomori K, Berns MW
Nucleic acids research 2009 May;37(9):e68

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Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity.

Guo Z, Zheng L, Dai H, Zhou M, Xu H, Shen B
Nucleic acids research 2009 Jun;37(10):3431-41

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Long patch base excision repair in mammalian mitochondrial genomes.

Szczesny B, Tann AW, Longley MJ, Copeland WC, Mitra S
The Journal of biological chemistry 2008 Sep 26;283(39):26349-56

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Comprehensive mapping of the C-terminus of flap endonuclease-1 reveals distinct interaction sites for five proteins that represent different DNA replication and repair pathways.

Guo Z, Chavez V, Singh P, Finger LD, Hang H, Hegde ML, Shen B
Journal of molecular biology 2008 Mar 28;377(3):679-90

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Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair.

Guo Z, Qian L, Liu R, Dai H, Zhou M, Zheng L, Shen B
Molecular and cellular biology 2008 Jul;28(13):4310-9

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Removal of oxidative DNA damage via FEN1-dependent long-patch base excision repair in human cell mitochondria.

Liu P, Qian L, Sung JS, de Souza-Pinto NC, Zheng L, Bogenhagen DF, Bohr VA, Wilson DM 3rd, Shen B, Demple B
Molecular and cellular biology 2008 Aug;28(16):4975-87

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HMGB1 is a cofactor in mammalian base excision repair.

Prasad R, Liu Y, Deterding LJ, Poltoratsky VP, Kedar PS, Horton JK, Kanno S, Asagoshi K, Hou EW, Khodyreva SN, Lavrik OI, Tomer KB, Yasui A, Wilson SH
Molecular cell 2007 Sep 7;27(5):829-41

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Condensin I interacts with the PARP-1-XRCC1 complex and functions in DNA single-strand break repair.

Heale JT, Ball AR Jr, Schmiesing JA, Kim JS, Kong X, Zhou S, Hudson DF, Earnshaw WC, Yokomori K
Molecular cell 2006 Mar 17;21(6):837-48

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Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay.

Shibata Y, Nakamura T
The Journal of biological chemistry 2002 Jan 4;277(1):746-54

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Direct interaction between mammalian DNA polymerase beta and proliferating cell nuclear antigen.

Kedar PS, Kim SJ, Robertson A, Hou E, Prasad R, Horton JK, Wilson SH
The Journal of biological chemistry 2002 Aug 23;277(34):31115-23

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: MCF7 10% SDS PAGE GTX70185 diluted at 1:500

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody (GTX70185) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed MCF7, using FEN1 [4E7](GTX70185) antibody at 1:200 dilution.