PA5-88244

antibody from Invitrogen Antibodies

Targeting: FEN1 FEN-1, MF1, RAD2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88244

Provider

Invitrogen Antibodies

Product name

FEN1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: GDVLQNEEGE TTSHLMGMFY RTIRMMENGI KPVYVFDGKP PQLKSGELAK RSERRAEAEK QLQQAQAAGA EQEVEKFTKR LVKVTKQHND ECKHLLSLMG IPYLDAPSEA EASCAALVKA GKVYAAATED MDCLTFGSPV LMRHLTASEA KKLPIQEFHL SRILQELGLN QEQFVDLCIL LGSDYCESIR GIGPKRAVDL IQKHKSIEEI VRRLDPNKYP VPENWLHKEA HQLFLEPEVL DPESVELKWS EPNEEELIKF MCGEKQFSEE RIRSGVKRLS KSRQGSTQGR LDDFFKVTGS LSSAKRKEPE PKGSTKKKAK TGAAGKFKRG K; Cellular Location: Mitochondrion, Nucleus, nucleolus, nucleoplasm

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.96 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of FEN1 in extracts of various cell lines using FEN1 Polyclonal Antibody (Product # PA5-88244) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of FEN1 was performed in A549 cells using FEN1 Polyclonal Antibody (Product # PA5-88244).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of FEN1 was performed in HeLa cells using FEN1 Polyclonal Antibody (Product # PA5-88244).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of FEN1 in paraffin-embedded human lung cancer using FEN1 Polyclonal Antibody (Product # PA5-88244) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of FEN1 in paraffin-embedded mouse liver using FEN1 Polyclonal Antibody (Product # PA5-88244) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of FEN1 was performed in 200 µg extracts of HeLa cells using FEN1 Polyclonal Antibody (Product # PA5-88244). Western blot was performed from the immunoprecipitate using FEN1 Polyclonal Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of FEN1 was performed in 200 µg extracts of HeLa cells using FEN1 Polyclonal Antibody (Product # PA5-88244). Western blot was performed from the immunoprecipitate using FEN1 Polyclonal Antibody.