PA5-79930

antibody from Invitrogen Antibodies

Targeting: RNF43 DKFZp781H0392, FLJ20315, URCC

Antibody data

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Validation data

Product number: PA5-79930 - Provider product page
Provider: Invitrogen Antibodies
Product name: RNF43 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 500 µg/mL
Storage: -20°C

RNF43 protein structure - PA5-79930 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RNF43 in Lane 1: human HeLa whole cell lysate, Lane 2: human 293T whole cell lysate, Lane 3: human COLO-320 whole cell lysate, Lane 4: human SW620 whole cell lysate, Lane 5: human MCF-7 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with RNF43 polyclonal antibody (Product # PA5-79930) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RNF43 in Lane 1: human HeLa whole cell lysate, Lane 2: human 293T whole cell lysate, Lane 3: human COLO-320 whole cell lysate, Lane 4: human SW620 whole cell lysate, Lane 5: human MCF-7 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with RNF43 polyclonal antibody (Product # PA5-79930) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.