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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- UM500084 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BBOX1 Monoclonal Antibody (UMAB119), UltraMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UMAB119
- Vial size
- 100 µL
- Concentration
- 0.5-1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15 µg) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Dilution: 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-BBOX1 mouse monoclonal antibody (UM500084, green, 1:50). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human breast duct using anti-BBOX1 mouse monoclonal antibody at 1:200 dilution of mg/mL using Polink2 Broad HRP DAB for detection. UM500084 requires HIER with citrate pH6.0 at 110°C for 3 min using pressure chamber/cooker. The image shows strong cytoplasmic, membrane, and nuclear staining in ductal epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human kidney carcinoma using anti-BBOX1 mouse monoclonal antibody at 1:200 dilution of mg/mL using Polink2 Broad HRP DAB for detection. UM500084 requires HIER with citrate pH6.0 at 110°C for 3 min using pressure chamber/cooker. The composit image shows strong cytoplasmic, membrane, and nuclear in panel A tumor cells and no staining in panel B tumor cells but strong staining is normal tubule epithelial cells..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human Kidney tissue using anti-BBOX1 mouse monoclonal antibody. (UM500084; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-BBOX1 mouse monoclonal antibody (UM500084). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
