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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
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- Product number
- PA5-18352 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Asporin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with rat based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Bone Marrow lysate using Product # PA5-18352 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Asporin by a Asporin monoclonal antibody (Product # PA5-18352) at a concentration of 0.1 µg/mL. Human Tonsil (A), (0.3 µg/mL) Human Uterus (B), Mouse Skeletal Muscle (C) (1 µg/mL) Rat Skeletal Muscle (D) and negative control Human Cerebellum (E) lysate (35µg protein in RIPA buffer).Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Bone Marrow lysate using Product # PA5-18352 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Asporin in HeLa cells using a Asporin monoclonal antibody (Product # PA5-18352) at 10 µg/mL for1hr. The cells were paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation was followed by Alexa Fluor 488 secondary antibody (4 µg/mL) showing nuclear membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL)followed by Alexa Fluor 488 secondary antibody (4 µg/mL).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of (A and C) ATDC5 cells and (B and C) DAPI using Product # PA5-18352.
