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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- PA1-27958 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MDMX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HepG2, MCF-7. Predicted reactivity: Cow: 80%; Dog: 93%; Horse: 93%; Pig: 86%; Rabbit: 80%. Use at a concentration of 1 µg/mL in 5% skim milk/PBS buffer. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MDM4 in HepG2 cell lysate using a MDM4 polyclonal antibody (Product # PA1-27958).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MDM4 in 10 µg MCF7 lysate using a MDM4 polyclonal antibody (Product # PA1-27958) and detected with an Anti-Rabbit HRP secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MDMX was performed by loading HepG2 cells. Proteins were transferred to a membrane and probed with a MDMX Polyclonal Antibody (Product # PA1-27958) at a dilution of 0.2-1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MDMX was performed by loading MCF-7 cells. Proteins were transferred to a membrane and probed with a MDMX Polyclonal Antibody (Product # PA1-27958) at a dilution of 1:1000.
