PA5-19473

antibody from Invitrogen Antibodies

Targeting: MDM4 HDMX, MDMX

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19473

Provider

Invitrogen Antibodies

Product name

MDMX Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA5-19473 targets MDMX in IF and WB applications and shows reactivity with Human samples.

Concentration

0.4 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using MDMX Polyclonal Antibody (Product # PA5-19473) and a 55 kDa band corresponding to MDMX/MDM4 was observed across cell lines tested and increased upon Serum starvation and Etoposide treatment in BeWo and IMR-32, also the expression was low in Mouse Heart. Modified whole cell extracts (1% SDS) (30 µg lysate) of BeWo (Lane 1), BeWo Serum starved cells (15 Hours) (Lane 2), BeWo treated with Etoposide (25 µM for 6 Hours) (Lane 3), IMR -32 (Lane 4), IMR -32 Serum starved cells (15 Hours) (Lane 5), IMR -32 treated with Etoposide (25 µM for 6 Hours) (Lane 6), OVCAR-3 (Lane 7), HEK-293 (Lane 8), as shown in Fig. a; tissue extracts (30 µg lysate) of Mouse Thymus (Lane 1) and Mouse Heart (Lane 2) as shown in Fig. b were electrophoresed using Novex® NuPAGE® 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).