TA505723

antibody from OriGene

Targeting: MDM4 HDMX, MDMX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505723

Provider

OriGene

Proper citation

OriGene Cat#TA505723, RRID:AB_2623319

Product name

MDM4 (MDMX) mouse monoclonal antibody, clone OTI4G5 (formerly 4G5)

Antibody type

Monoclonal

Description

MDM4 (MDMX) mouse monoclonal antibody, clone OTI4G5 (formerly 4G5)

Host

Mouse

Conjugate

Unconjugated

Epitope

MDM4

Isotype

IgG

Antibody clone number

OTI4G5

Vial size

100 µl

Concentration

0.6 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDM4 (RC209620, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDM4.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-MDM4 mouse monoclonal antibody (TA505723) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MDM4(RC209620).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MDM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505723)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MDM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505723)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MDM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505723)

Validation comment

IHC