TA505733

antibody from OriGene

Targeting: MDM4 HDMX, MDMX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505733

Provider

OriGene

Proper citation

OriGene Cat#TA505733, RRID:AB_2623328

Product name

MDM4 (MDMX) mouse monoclonal antibody, clone OTI4B10 (formerly 4B10)

Antibody type

Monoclonal

Description

MDM4 (MDMX) mouse monoclonal antibody, clone OTI4B10 (formerly 4B10)

Host

Mouse

Conjugate

Unconjugated

Epitope

MDM4

Isotype

IgG

Antibody clone number

OTI4B10

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MDM4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDM4 (RC209620, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDM4.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-MDM4 mouse monoclonal antibody (TA505733) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MDM4(RC209620).

Validation comment

IF