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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB6760 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6760, RRID:AB_10677661
- Product name
- MDM4 (phospho S367) monoclonal antibody, clone #15
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic phosphopeptide of MDM4.
- Isotype
- IgG
- Antibody clone number
- #15
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Induction of S367 phosphorylation after DNA damage is associated with increased binding of 14-3-3 to MDM4 and accelerated MDM4 degradation.MCF-7 cells were preincubated with the proteasome inhibitor MG132 (20 uM) and exposed to DNA damaging agent, adriamycin (3 uM) or etoposide (20 uM), for the indicated periods.
