Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- NBP2-30012 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal vH RAS Antibody
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 4.35 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: vH RAS Antibody [NBP2-30012] - HRAS (arrow) using rabbit polyclonal HRAS Antibody (C-term) (NBP2-30012). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HRAS gene.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: vH RAS Antibody [NBP2-30012] - Mouse cerebellum tissue lysates (35ug/lane).This demonstrates the HRAS antibody detected the HRAS protein (arrow).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: vH RAS Antibody [NBP2-30012] - HRAS Antibody (C-term)(NBP2-30012) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).