NBP2-42864

antibody from Novus Biologicals

Targeting: HRAS HRAS1

Immunocytochemistry

Immunoprecipitation

Antibody data

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Validation data

Product number: NBP2-42864 - Provider product page
Provider: Novus Biologicals
Product name: Rabbit Polyclonal HRAS Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified. This antibody is specific for H-Ras protein, and it does not cross react with human N-Ras or K-Ras protein.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

HRAS protein structure - NBP2-42864 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HRAS Antibody [NBP2-42864] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 12 % SDS-PAGE H-Ras antibody dilution: 1:1000

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HRAS Antibody [NBP2-42864] - Analysis of 30 ug whole cell lysate. A: Hela B: Hep G2 C: Molt-4 12% SDS PAGE diluted at 1:1000. .

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HRAS Antibody [NBP2-42864] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody diluted at 1:5000.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HRAS Antibody [NBP2-42864] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with H-Ras antibody diluted at 1:500.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HRAS Antibody [NBP2-42864] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HRAS Antibody [NBP2-42864] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HRAS antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.