Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [2]
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Validation data
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- Product number
- GTX61430 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX61430, RRID:AB_10621225
- Product name
- Ras antibody [EP1125Y], N-term
- Antibody type
- Monoclonal
- Antigen
- A synthetic peptide corresponding to residues near the N-terminal region of human Ras.
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µl
- Storage
- Store at -20°C. Stable for 12 months at -20°C
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- A. Western blot analysis on C6 cell lysate using anti-p21/Ras RabMAb (cat. # GTX61430).
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of C6 cell lysate using Ras antibody [EP1125Y] at a dilution of 1:500,000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of human Ras full length protein using Ras antibody [EP1125Y] at a dilution of 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- B. Flow cytometric analysis of permeabilized PC-12 cells using anti- p21/Ras (K and H Ras) RabMAb (red) (catalog #GTX61430) or a rabbit IgG (negative) (green).
- Validation comment
- FACS
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACS analysis of permeabilized PC-12 cells using Ras antibody [EP1125Y] at a dilution of 1:100 (red) and a IgG negative control (green).
- Validation comment
- FACS