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LearnMA5-14579
antibody from Invitrogen Antibodies
Targeting: FOLH1
FOLH, GCP2, GCPII, NAALAD1, NAALAdase, PSM, PSMA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-14579 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMA Recombinant Rabbit Monoclonal Antibody (SP29)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-14579 targets Prostate Specific Membrane Antigen in IHC (P) applications and shows reactivity with Human samples.
- Antibody clone number
- SP29
- Concentration
- 0.113 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of LNCaP (Lane 1), PC-3 (Lane 2) and DU 145 (Lane 3). The blot was probed with Rabbit Anti-PSMA Monoclonal Antibody (Product # MA5-14579, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 100 kDa band corresponding to PSMA was observed in LNCaP and not in PC-3 and DU 145 (negative controls). Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human prostate cancer stained with rabbit monoclonal PSMA using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining.
