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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- MA5-16250 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Dectin 2 Monoclonal Antibody (3D1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3D1
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Dectin 2 in human heart lysate. Samples were incubated in Dectin 2 monoclonal antibody (Product # MA5-16250) using a dilution of 2 µg/mL followed by a goat anti-mouse Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Dectin 2 in Normal human spleen tissue. Samples were incubated in Dectin 2 monoclonal antibody (Product # MA5-16250) using a dilution of 5 µg/mL followed by a peroxidase-conjugate and DAB chromogen. Human tissue TMA was used for this test.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Dectin 2 in THP-1 cells (blue) and a matched isotype control (orange). Samples were incubated in Dectin 2 monoclonal antibody (Product # MA5-16250) using a dilution of 5 µg/mL for 30 minutes at room temperature. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Dectin 2 in 5x10^5 human PBMCs. Samples were incubated in Dectin 2 monoclonal antibody (Product # MA5-16250) using a dilution of 0.5 µg. Antibody (red) and of isotype control antibody (0.5 µg) (green).
