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Learn22789-1-AP
antibody from Invitrogen Antibodies
Targeting: PHLPP1
KIAA0606, PHLPP, PLEKHE1, PPM3A, SCOP
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 22789-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHLPP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: QQHLLQVPA EASDEGIVIS ANEDEPGLPR KADFSAVGTI GRRRANGSVA PQERSHNVIE VATDAPLRKP GGYFAAPAQP DPDDQFIIPP ELEEEVKEIM KHHQEQQQQQ QPPPPPQLQP QLPRHYQLDQ LPDYYDTP (1068-1204 aa encoded by BC126277)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.57 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 22789-1-AP (PHLPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 22789-1-AP (PHLPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 22789-1-AP (PHLPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of PHLPP antibody (22789-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLPP transfected HeLa cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHLPP antibody (Product # 22789-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using PHLPP antibody 22789-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver slide using 22789-1-AP (PHLPP Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver slide using 22789-1-AP ( PHLPP Antibody) at dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PHLPP (IP:22789-1-AP, 4ug; Detection:22789-1-AP 1:600) with mouse brain tissue lysate 4000 ug.
