22789-1-AP

antibody from Proteintech Group

Targeting: PHLPP1 KIAA0606, PHLPP, PLEKHE1, PPM3A, SCOP

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

22789-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#22789-1-AP, RRID:AB_2750897

Product name

PHLPP antibody

Antibody type

Polyclonal

Description

KD/KO validated PHLPP antibody (Cat. #22789-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Reduced PHLPP Expression Leads to EGFR-TKI Resistance in Lung Cancer by Activating PI3K-AKT and MAPK-ERK Dual Signaling.

Wang W, Xia X, Chen K, Chen M, Meng Y, Lv D, Yang H
Frontiers in oncology 2021;11:665045

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Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.

Suryawan A, El-Kadi SW, Nguyen HV, Fiorotto ML, Davis TA
The Journal of nutrition 2021 Sep 4;151(9):2636-2645

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Basolateral protein Scribble binds phosphatase PP1 to establish a signaling network maintaining apicobasal polarity.

Troyanovsky RB, Indra I, Kato R, Mitchell BJ, Troyanovsky SM
The Journal of biological chemistry 2021 Nov;297(5):101289

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The PHLPP1 N-Terminal Extension is a Mitotic Cdk1 Substrate and Controls an Interactome Switch.

Kawashima AT, Wong C, Lordén G, King CC, Lara-Gonzalez P, Desai A, Gingras AC, Newton AC
Molecular and cellular biology 2021 Jan 4;

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CUL4B negatively regulates Toll-like receptor-triggered proinflammatory responses by repressing Pten transcription.

Song Y, Li P, Qin L, Xu Z, Jiang B, Ma C, Shao C, Gong Y
Cellular & molecular immunology 2021 Feb;18(2):339-349

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Inhibition of PHLPP1/2 phosphatases rescues pancreatic β-cells in diabetes.

Lupse B, Annamalai K, Ibrahim H, Kaur S, Geravandi S, Sarma B, Pal A, Awal S, Joshi A, Rafizadeh S, Madduri MK, Khazaei M, Liu H, Yuan T, He W, Gorrepati KDD, Azizi Z, Qi Q, Ye K, Oberholzer J, Maedler K, Ardestani A
Cell reports 2021 Aug 3;36(5):109490

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Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

He X, Li M, Yu H, Liu G, Wang N, Yin C, Tu Q, Narla G, Tao Y, Cheng S, Yin H
PLoS biology 2020 Dec;18(12):e3000803

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Cigarette smoke extract induces airway epithelial cell death via repressing PRMT6/AKT signaling.

Li T, Fanning KV, Nyunoya T, Chen Y, Zou C
Aging 2020 Dec 1;12(23):24301-24317

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Inhibition of PHLPP1 ameliorates cardiac dysfunction via activation of the PI3K/Akt/mTOR signalling pathway in diabetic cardiomyopathy.

Zhang M, Wang X, Liu M, Liu D, Pan J, Tian J, Jin T, Xu Y, An F
Journal of cellular and molecular medicine 2020 Apr;24(8):4612-4623

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USP1 deubiquitinates Akt to inhibit PI3K-Akt-FoxO signaling in muscle during prolonged starvation.

Goldbraikh D, Neufeld D, Eid-Mutlak Y, Lasry I, Gilda JE, Parnis A, Cohen S
EMBO reports 2020 Apr 3;21(4):e48791

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Phlpp1 is associated with human intervertebral disc degeneration and its deficiency promotes healing after needle puncture injury in mice.

Zhang C, Smith MP, Zhou GK, Lai A, Hoy RC, Mroz V, Torre OM, Laudier DM, Bradley EW, Westendorf JJ, Iatridis JC, Illien-Jünger S
Cell death & disease 2019 Oct 3;10(10):754

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PHLPP1 counter-regulates STAT1-mediated inflammatory signaling.

Cohen Katsenelson K, Stender JD, Kawashima AT, Lordén G, Uchiyama S, Nizet V, Glass CK, Newton AC
eLife 2019 Aug 13;8

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Protein Kinase C Quality Control by Phosphatase PHLPP1 Unveils Loss-of-Function Mechanism in Cancer.

Baffi TR, Van AN, Zhao W, Mills GB, Newton AC
Molecular cell 2019 Apr 18;74(2):378-392.e5

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PHLPP regulates hexokinase 2-dependent glucose metabolism in colon cancer cells.

Xiong X, Wen YA, Mitov MI, C Oaks M, Miyamoto S, Gao T
Cell death discovery 2017;3:16103

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High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.

Xie Y, Lv D, Wang W, Ye M, Chen X, Yang H
Oncotarget 2017 Aug 29;8(35):59000-59007

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Pleckstrin Homology (PH) Domain Leucine-rich Repeat Protein Phosphatase Controls Cell Polarity by Negatively Regulating the Activity of Atypical Protein Kinase C.

Xiong X, Li X, Wen YA, Gao T
The Journal of biological chemistry 2016 Nov 25;291(48):25167-25178

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PHLPP negatively regulates cell motility through inhibition of Akt activity and integrin expression in pancreatic cancer cells.

Smith AJ, Wen YA, Stevens PD, Liu J, Wang C, Gao T
Oncotarget 2016 Feb 16;7(7):7801-15

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Loss of PHLPP protects against colitis by inhibiting intestinal epithelial cell apoptosis.

Wen YA, Li X, Goretsky T, Weiss HL, Barrett TA, Gao T
Biochimica et biophysica acta 2015 Oct;1852(10 Pt A):2013-23

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse brain tissue were subjected to SDS PAGE followed by western blot with 22789-1-AP(PHLPP antibody) at dilution of 1:1000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PHLPP antibody 22789-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-PHLPP (IP:22789-1-AP, 5ug; Detection:22789-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

Sample type

tissue