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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF856 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-18R alpha/IL-1 R5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IL-18 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human IL-18 R is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Postnatal changes of interleukin-18 receptor immunoreactivity in neurons of the retrosplenial cortex in wild-type and interleukin-18 knock out mice.
Interleukin-18 and its receptor are expressed in gonadotropin-releasing hormone neurons of mouse and rat forebrain.
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.
Interleukin-18 suppresses adiponectin expression in 3T3-L1 adipocytes via a novel signal transduction pathway involving ERK1/2-dependent NFATc4 phosphorylation.
Protective effect of IL-18 on kainate- and IL-1 beta-induced cerebellar ataxia in mice.
Protective effect of IL-18 on kainate- and IL-1 beta-induced cerebellar ataxia in mice.
IL-18 induces a marked gene expression profile change and increased Ccl1 (I-309) production in mouse mucosal mast cell homologs.
Disruption of interleukin-18, but not interleukin-1, increases vulnerability to preterm delivery and fetal mortality after intrauterine inflammation.
Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth.
Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
Hayakawa T, Hata M, Kuwahara-Otani S, Yagi H, Okamura H
Okajimas folia anatomica Japonica 2017;94(3):93-99
Okajimas folia anatomica Japonica 2017;94(3):93-99
Interleukin-18 and its receptor are expressed in gonadotropin-releasing hormone neurons of mouse and rat forebrain.
Kuwahara-Otani S, Maeda S, Kobayashi K, Minato Y, Tanaka K, Yamanishi K, Hata M, Li W, Hayakawa T, Noguchi K, Okamura H, Yagi H
Neuroscience letters 2017 May 22;650:33-37
Neuroscience letters 2017 May 22;650:33-37
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.
Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11
Interleukin-18 suppresses adiponectin expression in 3T3-L1 adipocytes via a novel signal transduction pathway involving ERK1/2-dependent NFATc4 phosphorylation.
Chandrasekar B, Patel DN, Mummidi S, Kim JW, Clark RA, Valente AJ
The Journal of biological chemistry 2008 Feb 15;283(7):4200-9
The Journal of biological chemistry 2008 Feb 15;283(7):4200-9
Protective effect of IL-18 on kainate- and IL-1 beta-induced cerebellar ataxia in mice.
Andoh T, Kishi H, Motoki K, Nakanishi K, Kuraishi Y, Muraguchi A
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2322-8
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2322-8
Protective effect of IL-18 on kainate- and IL-1 beta-induced cerebellar ataxia in mice.
Andoh T, Kishi H, Motoki K, Nakanishi K, Kuraishi Y, Muraguchi A
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2322-8
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2322-8
IL-18 induces a marked gene expression profile change and increased Ccl1 (I-309) production in mouse mucosal mast cell homologs.
Wiener Z, Pocza P, Racz M, Nagy G, Tolgyesi G, Molnar V, Jaeger J, Buzas E, Gorbe E, Papp Z, Rigo J, Falus A
International immunology 2008 Dec;20(12):1565-73
International immunology 2008 Dec;20(12):1565-73
Disruption of interleukin-18, but not interleukin-1, increases vulnerability to preterm delivery and fetal mortality after intrauterine inflammation.
Wang X, Hagberg H, Mallard C, Zhu C, Hedtjärn M, Tiger CF, Eriksson K, Rosen A, Jacobsson B
The American journal of pathology 2006 Sep;169(3):967-76
The American journal of pathology 2006 Sep;169(3):967-76
Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth.
Chaudhry UI, Kingham TP, Plitas G, Katz SC, Raab JR, DeMatteo RP
Cancer research 2006 Nov 1;66(21):10497-504
Cancer research 2006 Nov 1;66(21):10497-504
Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1754-62
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1754-62
Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1754-62
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1754-62
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- IFN-gamma Secretion Induced by IL-18/IL-1F4 and Neutralization by Mouse IL-18 R alpha/IL-1 R5 Antibody. In the presence of Recombinant Mouse IL-12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 R alpha/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856). The ND50 is typically 0.2-0.8 µg/mL.
