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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB840-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-18 R alpha/IL-1 R5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects Recombinant Human IL-18 R alpha/IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q13478- Isotype
- IgG
- Antibody clone number
- 70625
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma.
Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter.
IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures.
Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
CD molecules 2006--human cell differentiation molecules.
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Lin L, Wang J, Liu D, Liu S, Xu H, Ji N, Zhou M, Zeng X, Zhang D, Li J, Chen Q
Scientific reports 2016 May 26;6:26757
Scientific reports 2016 May 26;6:26757
Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter.
Wang J, Sun C, Gerdes N, Liu C, Liao M, Liu J, Shi MA, He A, Zhou Y, Sukhova GK, Chen H, Cheng XW, Kuzuya M, Murohara T, Zhang J, Cheng X, Jiang M, Shull GE, Rogers S, Yang CL, Ke Q, Jelen S, Bindels R, Ellison DH, Jarolim P, Libby P, Shi GP
Nature medicine 2015 Jul;21(7):820-6
Nature medicine 2015 Jul;21(7):820-6
IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures.
Blom L, Poulsen LK
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4331-7
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4331-7
Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H
Arthritis research & therapy 2011 Mar 24;13(2):R52
Arthritis research & therapy 2011 Mar 24;13(2):R52
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
Rao DA, Tracey KJ, Pober JS
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6536-46
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6536-46
CD molecules 2006--human cell differentiation molecules.
Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Bühring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
Journal of immunological methods 2007 Jan 30;319(1-2):1-5
Journal of immunological methods 2007 Jan 30;319(1-2):1-5
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Ebert EC, Mehta V
Cellular immunology 2006 May;241(1):7-13
Cellular immunology 2006 May;241(1):7-13
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-18 R alpha/IL-1 R5 in Human PBMCs. IL-18 R alpha/IL-1 R5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human IL-18 R alpha/IL-1 R5 Mono-clonal Antibody (Catalog # MAB840) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IFN-gamma Secretion Induced by IL-18/IL-1F4 and Neutrali-zation by IL-18 R alpha/IL-1 R5 Antibody. In the presence of Recombinant Human TNF-alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL-18/IL-1F4 (Catalog # B003-5) stimulates IFN-gamma secretion in KG-1 human myeloid leukemia cells in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/IL-1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-18 R alpha/ IL-1 R5 Monoclonal Antibody (Catalog # MAB840). The ND50 is typically 0.03-0.1 µg/mL.
