Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 329716 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#329716, RRID:AB_11149168
- Product name
- Ultra-LEAF™ Purified anti-human CD274 (B7-H1, PD-L1) [29E.2A3]
- Antibody type
- Monoclonal
- Antigen
- Full length human PD-L1
- Description
- CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-g production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- 1 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with purified CD274 (clone 29E.2A3) (filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG PE.
- Conjugate
- Unconjugated