NB300-903

antibody from Novus Biologicals

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-903

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-903, RRID:AB_10003509

Product name

Goat Polyclonal PD-L1 Antibody

Antibody type

Polyclonal

References

Submitted references

Costimulatory B7-H1 in renal cell carcinoma patients: Indicator of tumor aggressiveness and potential therapeutic target.

Thompson RH, Gillett MD, Cheville JC, Lohse CM, Dong H, Webster WS, Krejci KG, Lobo JR, Sengupta S, Chen L, Zincke H, Blute ML, Strome SE, Leibovich BC, Kwon ED
Proceedings of the National Academy of Sciences of the United States of America 2004 Dec 7;101(49):17174-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PD-L1/B7-H1 Antibody [NB300-903] - Staining of Human Heart (A) lysate + Blocking peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NB300-903] - B7-H1/PD-L1/CD274 Antibody [NB300-903] - (2ug/ml) staining of paraffin embedded Human Placenta. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NB300-903] - Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: PD-L1/B7-H1 Antibody [NB300-903] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.