AP16317PU-N

antibody from Acris Antibodies GmbH

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP16317PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP16317PU-N, RRID:AB_1928278

Product name

anti CD274 / PDL1 (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide from the C-Terminus of Human (NP_054862.1)

Reactivity

Human

Host

Goat

Vial size

0.1 mg

Concentration

0.5 mg/ml

References

Submitted references

Upregulation of B7-H1 expression is associated with macrophage infiltration in hepatocellular carcinomas.

Chen J, Li G, Meng H, Fan Y, Song Y, Wang S, Zhu F, Guo C, Zhang L, Shi Y
Cancer immunology, immunotherapy : CII 2012 Jan;61(1):101-8

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP16317PU-N staining (0.3 µg/ml) of Human Heart lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP16317PU-N (0.3 µg/ml) staining of Human placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.chemiluminescence

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP16317PU-N (4 µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP16317PU-N (4 µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.