AF783

antibody from R&D Systems

Targeting: CD70 CD27L, CD27LG, TNFSF7

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF783

Provider

R&D Systems

Product name

Mouse CD27 Ligand/TNFSF7 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse CD27 Ligand in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human CD27 Ligand is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

O55237


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

HIV-1 adenoviral vector vaccines expressing multi-trimeric BAFF and 4-1BBL enhance T cell mediated anti-viral immunity.

Kanagavelu S, Termini JM, Gupta S, Raffa FN, Fuller KA, Rivas Y, Philip S, Kornbluth RS, Stone GW
PloS one 2014;9(2):e90100

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by CD27 Ligand/TNFSF7 and Neutralization by Mouse CD27 Ligand/TNFSF7 Anti-body. In the presence of sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484), Recombinant Mouse CD27 Ligand/TNFSF7 (Catalog # 783-CL) stimulates proliferation in mouse splenic T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Mouse CD27 Ligand/TNFSF7 (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CD27 Ligand/TNFSF7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF783). The ND₅₀ is typically 3-12 µg/mL.