PA5-46799

antibody from Invitrogen Antibodies

Targeting: ANKRD17 FLJ22206, GTAR, KIAA0697, MASK2, NY-BR-16

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46799

Provider

Invitrogen Antibodies

Product name

ANKRD17 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: PRGMVRVCDL LLKKKPPQQQ HHKAKRNRTC RPPSSSESSS DSDNSGGGGG

Concentration

0.5 mg/mL

References

Submitted references

Functional Analyses of Bovine Foamy Virus-Encoded miRNAs Reveal the Importance of a Defined miRNA for Virus Replication and Host-Virus Interaction.

Cao W, Stricker E, Hotz-Wagenblatt A, Heit-Mondrzyk A, Pougialis G, Hugo A, Kuźmak J, Materniak-Kornas M, Löchelt M
Viruses 2020 Nov 2;12(11)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human 293T whole cell lysate using an anti-ANKRD17 polyclonal antibody (Product # PA5-46799).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 8 Validation of steady-state protein levels of ANKRD17 ( A ) and Bif1 ( B ) as direct targets of miR-BF2-5p. MDBK cells were transfected with miR-BF2-5p mimic and mimic NC (negative control), or U6 promoter driven cassette and U6 promoter empty plasmid (NC), or infected with wt BFV-MDBK24 and DeltamiRNA BFV-MDBK24. After three days, the transfected and infected cells were harvested and protein was extracted for western blotting. The expression of beta-actin was monitored as a control for proper protein loading. In this blot representing one out of two experiments, the 75 kDa splice variant of bovine ANKRD17 and the full-length 40 kDa Bif1 proteins were specifically detected using antibodies as described in Section 2.7 .