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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX129444 - Provider product page
- Provider
- GeneTex
- Product name
- ARID2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- ARID2 antibody detects ARID2 protein by western blot analysis.A. 30 ?g 293T B. 30 ?g of human ARID2-transfected 293T cells5% SDS-PAGEARID2 antibody (GTX129444) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
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- Experimental details
- ARID2 antibody detects ARID2 protein by western blot analysis.A. 30 ?g HeLa whole cell lysate/extract B. 30 ?g HeLa nuclear lysate/extract5% SDS-PAGEARID2 antibody (GTX129444) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ARID2 antibody detects ARID2 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129444) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ARID2 antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129444) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- ARID2 antibody detects ARID2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ARID2 protein stained by ARID2 antibody (GTX129444) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- ARID2 antibody immunoprecipitates ARID2 protein in IP experiments.IP samples: HepG2 whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of ARID2 protein by 4 £gg ARID2 antibody (GTX129444)5 % SDS-PAGEThe immunoprecipitated ARID2 protein was detected by ARID2 antibody (GTX129444) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
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- GeneTex (provider)
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- Experimental details
- ARID2 antibody detects ARID2 protein on human hepatoma and human normal liver by immunohistochemical analysis. Sample: (a) Paraffin-embedded human hepatoma (b) Paraffin-embedded human normal liver. ARID2 antibody (GTX129444) dilution: 1:1000.
