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LearnA302-230A
antibody from Invitrogen Antibodies
Targeting: ARID2
BAF200, DKFZp686G052, FLJ30619, KIAA1557
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- A302-230A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARID2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ARID2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID2 antibody (Product # A302-230A; lot A302-230A-2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ARID2 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARID2 antibody A302-230A used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. ARID2 was also immunoprecipitated by rabbit anti-ARID2 antibody A302-229A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ARID2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID2 (Product # A302-230A; lot 2) used at a dilution of 1:5,000 ( 0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ARID2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ARID2 (Product # A302-230A lot 2) used at a dilution of 1:5,000 ( 0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ARID2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARID2 (Cat. No. A302-230A) used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ARID2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID2 antibody (Product # A302-230A; lot A302-230A-2) used for IP at 6 µg per reaction. ARID2 was also immunoprecipitated by a previous lot of this antibody (lot A302-230A-1). For blotting immunoprecipitated ARID2 (Product # A302-230A) was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
