15578-1-AP

antibody from Invitrogen Antibodies

Targeting: ALDH3A1 ALDH3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15578-1-AP

Provider

Invitrogen Antibodies

Product name

ALDH3A1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LGVVLVIGT WNYPFNLTIQ PMVGAIAAGN AVVLKPSELS ENMASLLATI IPQYLDKDLY PVINGGVPET TELLKERFDH ILYTGSTGVG KIIMTAAAKH LTPVTLELGG KSPCYVDKNC DLDVACRRIA WGKFMNSGQT CVAPDYILCD PSIQNQIVEK LKKSLKEFYG EDAKKSRDYG RIISARHFQR VMGLIEGQKV AYGGTGDAAT RYIAPTILTD VDPQSPVMQE EIFGPVLPIV CVRSLEEAIQ FINQREKPLA LYMFSSNDKV IKKMIAETSS GGVAANDVIV HITLHSLPFG GVGNSGMGSY HGKKSFETFS HRRSCLVRPL MNDEGLKVRY PPSPAKMTQH (105-453 aa encoded by BC004370)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.18 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 15578-1-AP (ALDH3A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 15578-1-AP (ALDH3A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using ALDH3A1 antibody 15578-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HEK-293 cells were intracellularly stained with 0.25 µg Anti-Human ALDH3A1 (Product # 15578-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.25 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ALDH3A1 (IP:15578-1-AP, 4ug; Detection:15578-1-AP 1:1000) with HEK-293 cells lysate 3200ug.